Adaptation of hybrid immunoaffinity LC–MS methods for protein bioanalysis in a Contract Research Organization
B Jones & G A Schultz | Bioanalysis, 8(15), 1545-1549 (2016)
Keywords: • high-resolution MS (HRMS) • immunoaffinity (IA) • immunoprecipitation (IP) • microflow chromatography • nanoflow chromatography • sequential immunoprecipitation (sequential-IP) • signature peptide • surrogate peptide
Incorporation of immunoaffinity (IA)-based enrichment approaches to LC–MS detection technologies has led to many advancements in bioanalysis of protein therapeutics and biomarkers. The added specificity of antibody–antigen interactions has revealed new potential in LC–MS techniques, and the added degrees of selectivity that LC–MS detection strategies provide can overcome challenges encountered in traditional ligand-binding workflows. The new bioanalytical tools provided by the combination of these two disciplines promise solutions to challenges of modern drug development, as we seek to understand more of the molecular dynamics related to drug metabolism, target engagement and antidrug–antibody interactions.
