Measuring Cell Division in PMBCs by Flow Cytometry

Written by Biotium

The level of cell proliferation provides insights into relative cell health and is a widely used diagnostic indicator for numerous cancer types. In immunology, measuring cell proliferation is particularly useful for assessing immune responses such as T cell activation. In the past, cell proliferation assays involved radioactive labelling of DNA, but today fluorescent dyes such as ViaFluor® SE and CFSE are a safer and easier alternative for tracking cell replication in vivo and in vitro 

Cell proliferation of peripheral blood mononuclear cells (PMBCs) can be a useful read-out of immune cell activation. In their latest Tech Tip, Biotium outlines a typical procedure for using fluorescent ViaFluor® SE Dyes with PMBCs, which can easily be adapted for use with other cell types. 

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In this Tech Tip, you will learn:

  • A typical procedure for using fluorescent ViaFluor® SE dyes for measuring lymphocyte proliferation in peripheral blood mononuclear cells.
  • Key tips for getting the best results from your assays
  • Key considerations for proliferation dye and viability stain selection

And much more!

This content was provided by Biotium