One instrument, multiple jobs: analysis of biomolecules using Q-TOF HRMS
Tuesday 16 November 2021
07:00 [PST] 10:00 [EST] 15:00 [GMT]
This webinar introduces the application of Q-TOF HRMS in the analysis of biomolecules with a wide range of molecular weights. The first section demonstrates the comparable sensitivity, linearity and multiplexity between HRMS and triple quadrupole when quantitating a panel of peptide hormones (MW 1 kDa to 9.4 kDa). The advantages of HRMS over triple quadrupole in targeted quantitation are also discussed.
The second section illustrates how Q-TOF is used to perform native MS analysis without instrumentation modifications. With optimized instrumental settings, noncovalent protein complexes up to 450 kDa can be characterized.
What will you learn?
- Sensitivity, selectivity, linearity and multiplexity of Q-TOF HRMS in targeted peptide quantitation
- The advantages and disadvantages of Q-TOF HRMS for quantitative purpose when compared with triple quadrupole MS
- How to optimize instrument settings of Q-TOF HRMS to perform native MS analysis without hardware modifications
- How in vitro noncovalent protein complexes are characterized by native MS
- The technical limitations of commercially available Q-TOF HRMS to analyze macromolecules in the native state
Who may this interest?
- Scientists seeking to develop LC−MS assays for PK/PD analysis with high selectivity and sensitivity
- Lab managers who consider investing a versatile mass spectrometer capable of analyzing a wide range of biomolecules
- Researchers with limited instrument availability but who are interested in analyzing noncovalent protein-protein interactions or characterizing protein complexes by native MS
The winner of the Bioanalysis Rising Star Award 2021 – announced soon!
Exploratory Biomaker Assay Sr. Analyst
GSK (PA, USA)
Zhuo Chen received his BS and MS degree in Peking University (Beijing, China) and then pursued a PhD degree at Brown University (RI, USA) focusing on mass spectrometry-based proteomics. After graduating from Brown in 2016, Zhuo joined GSK as a GSK/Waters joint postdoctoral fellow. In the department of bioanalysis, immunogenicity and biomarkers (BIB), his work focused on developing LC−MS assays to quantitate protein therapeutics and biomarkers. Since switching to a full-time GSK employee of BIB in 2017, he took more responsibility for supporting GLP-regulated PK/PD assays. Zhuo has published five bioanalytical papers since 2019 and delivered oral presentations at ASMS and CPSA annual conferences.
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