Publication / Source: Bioanalysis 1(4)
Authors: Abdel-Rehim M
“A good sample-preparation method will effectively isolate the analyte of interest from a complex matrix for quantification.”
Sample preparation is an important step in the bioanalysis process because of the difficulties that follow when using complex sample matrices, such as plasma and urine. A good sample-preparation method will effectively isolate the analyte of interest from a complex matrix for quantification. It will enhance selectivity and sensitivity, and eliminate the ion suppression, caused by the matrix, in ESI–MS. For the extraction of drugs from urine and plasma matrices, liquid–liquid extraction (LLE) methods require solvent or solvent mixtures of quite high polarity, and these often yield emulsions and a high matrix background in the extracted sample.