Bioanalysis Zone

Bioanalytical method for in vitro metabolism study of repaglinide using 96-blade thin-film solid-phase microextraction and LC–MS/MS

0

Background: A high-throughput bioanalytical method using 96-blade thin film microextraction (TFME) and LC–MS/MS for the analysis of repaglinide (RPG) and two of its main metabolites was developed and used for an in vitro metabolism study. Results: The target analytes were extracted from human microsomal medium by a 96-blade-TFME system employing the low-cost prototype ‘SPME multi-sampler’ using C18 coating. Method validation showed recoveries around 90% for all analytes and was linear over the concentration range of 2–1000 ng ml-1 for RPG and of 2–500 ng ml-1 for each RPG metabolite. Conclusion: The method was applied to an in vitro metabolism study of RPG employing human liver microsomes and proved to be very useful for this purpose.

To view restricted content, please:
Share:

Leave A Comment

Please wait...

Bioanalysis Zone New Investigator Award

Why not start this year by doing something good by nominating an early career scientist for the Bioanalysis Zone New Investigator Award. Complete the details below to register your interest!