In this Technology Brief the authors describe a robust and reproducible pellet digestion and LC-MS/MS methodology to quantify the mAb trastuzumab. A simple PPT clean-up and post-pellet digestion using the ProteinWorks eXpress Direct Digest Kit yielded an accurate, precise and robust LC-MS quantification of trastuzumabvia the surrogate peptide approach.
Area counts for trastuzumab peptides increased 2–8x using the above strategy, significantly improving sensitivityand specificity, whilst achieving accurate and reproducible quantitative results.
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