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Critical reagent screening and characterization: benefits and approaches for protein biomarker assays by hybrid LC–MS

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In this perspective article from Bioanalysis, the authors discuss the similarities and differences of critical reagents in LBAs and hybrid LC–MS assays. The authors propose that, although use of hybrid LBA/LC–MS assays has increased in recent years, unlike for LBAs alone, the benefits of well-characterized hybrid LC–MS assay reagents are frequently underestimated.

Keywords: endogenous biomarker, critical reagents, hybrid LC–MS assay, immunocapture, LBA, surrogate peptide

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