Development of Native and Denaturing LC-MS Methods for Characterization of Cysteine-Linked Antibody Drug Conjugates Using a Non-Toxic Mimic

Here we utilize a cysteine-linked antibody fluorophore conjugate to show how LC-MS methods may be optimized using a non-toxic ADC-mimic.  The ADC-mimic was used to develop native and reduced SEC-MS conditions. The reduced mimic’s heavy and light chains, as well as its IdeS-proteolysis fragments, were used to develop reversed-phase LC-MS methods.  A tryptic digest of the ADC mimic was used to develop a peptide mapping LC-MS method.

Download Poster Now

Find out more about the Life Science business of Merck KGaA and their products here.