Beyond the Abstract: an interview with Gaudry Troché

Interviews Microsampling MS (LC–MS/MS, HRMS, IMS)

In our new podcast series, we’re joined by a Bioanalysis author to review their publication’s biggest challenges, wider implications and next steps. In episode two, Digital Editor Ellen Williams is joined by Research Assistant Gaudry Troché to discuss conventional sampling and microsampling for the comparison of pharmacokinetic study profiles of insulin. Gaudry talks about life as an early career scientist, his plans for the future and his poster presentation at the Health Benefits and Leadership conference in Las Vegas (HBLC; NV, USA).

The Bioanalysis article:

Comparison of pharmacokinetic study profiles of insulin in rat plasma through conventional sampling and microsampling by micro-LC–MS/MS

Troché GB, Søeborg T, Bödvarsdottir TB, Bjelke M  & Nielsen NJ | Bioanalysis, 15(5), 283–294, (2023)

Keywords: • insulins • microflow liquid chromatography • microsampling • pharmacokinetics

Aim: With microsamples of blood, full pharmacokinetic profiles from individual animals can be obtained as an alternative to the sparse-sampling approach, where conventional volume samples from several animals are required. However, microsamples require assays that are more sensitive. Methods: The sensitivity of the LC–MS assay was increased 47-fold using microflow LC–MS. Results & conclusion: By analyzing both microsamples and conventional samples from the same animals, it is demonstrated that sparse-sampling profiles can be nonrepresentative of the full profiles. This bias can affect the tested treatment by increasing or reducing its apparent effect. Microsampling enables unbiased results compared with sparse-sampling. An increase in assay sensitivity to balance the low sample volumes was achievable by microflow LC–MS.

Read the full Research Article via Bioanalysis

 

Questions

  1. Could you provide a brief introduction to the paper? [0:32]
  2. What were the key challenges you faced during the project? Were there any failed stages that did not make it into the paper? [2:02]
  3. What was it like being an early career scientist in your lab? [3:17]
  4. What are your plans for the future following on from this publication? [4:08]
  5. What can an audience expect to see from your poster at HBLC 2023? [5:13]

 

About the speaker:

Gaudry Troché
Research Assistant
University of Copenhagen (Denmark)

Gaudry is a Research Assistant working at the Globe Institute and the Center for Protein Research (CPR) at the University of Copenhagen. He graduated from the University of Copenhagen in Analytical Chemistry in 2022, with two articles published as the first author. Gaudry specializes in LC-MS-based analysis and his current position involves technical, routine operation and development of MS-based proteomics, with a particular focus on sample preparation. Having a strong interest in chromatography, Gaudry plans to start a PhD in multidimensional liquid chromatography within the next 5 years.

 

Listen to Episode one of this series here.