Review on in vivo profiling of drug metabolites with LC-MS/MS in the past decade


Reddy GN, Laltanpuii C, Sonti R | Bioanalysis, 13(22), 1697–1722 (2021) Keywords: biotransformation • LC–MS • metabolite profiling • plasma • urine Metabolite profiling is an indispensable part of drug discovery and development, enabling a comprehensive understanding of the drug's metabolic behavior. Liquid chromatography-mass spectrometry facilitates metabolite profiling by reducing sample complexity and providing high sensitivity. This review discusses the in vivo metabolite profiling involving LC-MS/MS and the utilization of QTOF, QQQ mass analyzers with a particular emphasis on a mass filter. Further, a summary of sample extraction procedures in biological matrices such as plasma, urine, feces, serum and hair as in vivo samples are outlined. toward...

To view this content, please register now for access

It's completely free