Bioanalysis Zone

Improving selectivity and sensitivity of protein quantitation by LC–HR–MS/MS: determination of somatropin in rat plasma

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Aim: Protein quantitation by digestion of a biological sample followed by LC–MS analysis of a signature peptide can be a challenge because of the high complexity of the digested matrix.

Results/methodology: The use of LC with high-resolution (quadrupole-TOF) MS detection allowed

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