Advanced LC-MS applications for proteomics
Editor: Stephen R Pennington (University College Dublin)
In this chapter, protein digestion, solid-phase extraction and affinity purification are outlined, and considerations prior to sample preparation are proposed.
In this chapter, the basic concepts and major advances in current LC separation technologies for proteomics are introduced and discussed.
In this chapter the challenges associated with proteomic analysis of bio-fluids and the recent progress in identifying potential biomarkers within the major urological cancers are detailed. The steps necessary to translate these preliminary findings into eventual clinical application are also outlined.
In this chapter an up-to-date summary of the major techniques currently used by researchers to measure global protein phosphorylation levels are presented, highlighting their applications in the study of emergent biological processes.
In this chapter a selection of peptide quantitation techniques, many of which involve labeling proteins with heavy and light stable-isotope pairs, are described.
In this chapter the current state of the proteome catalogues of the major human biofluids of plasma, urine, liquor and saliva are reviewed. Large-scale plasma proteome studies that provide promising approaches to bridge the gap from biomarker discovery towards clinical practice are also presented.