ISBN: 978-1-910419-18-2

Editor: Stephen R Pennington (University College Dublin)


Chapter 1: Automated sample preparation strategies for high-throughput analysis of body fluids

In this chapter, protein digestion, solid-phase extraction and affinity purification are outlined, and considerations prior to sample preparation are proposed.


Chapter 2: Advances in LC separations for proteomics

In this chapter, the basic concepts and major advances in current LC separation technologies for proteomics are introduced and discussed.


Chapter 3: Proteomic approaches to biomarker discovery for prostate, bladder and kidney cancers

In this chapter the challenges associated with proteomic analysis of bio-fluids and the recent progress in identifying potential biomarkers within the major urological cancers are detailed. The steps necessary to translate these preliminary findings into eventual clinical application are also outlined.


Chapter 4: Advanced applications for identification and quantification of the phosphoproteome

In this chapter an up-to-date summary of the major techniques currently used by researchers to measure global protein phosphorylation levels are presented, highlighting their applications in the study of emergent biological processes.


Chapter 5: Modern techniques in quantitative proteomics

In this chapter a selection of peptide quantitation techniques, many of which involve labeling proteins with heavy and light stable-isotope pairs, are described.


Chapter 6: Personalized proteomics of human biofluids for clinical applications

In this chapter the current state of the proteome catalogues of the major human biofluids of plasma, urine, liquor and saliva are reviewed. Large-scale plasma proteome studies that provide promising approaches to bridge the gap from biomarker discovery towards clinical practice are also presented.