In recent years, researchers have thoroughly investigated the use of immunoaffinity techniques for sample purification before digestion. Compared to typical ELISA optical based techniques, hyphenated immunocapture-LC–MS/MS boasts a wider dynamic range and excellent robustness and reproducibility.
This poster from SCIEX (Warrington, UK) reports the use of an immunoaffinity high-resolution accurate mass assay as a complementary analytical technique for signature peptide quantification. To learn more about the assay, including the limits of detection and quantification and the potential to meet the demands of preclinical bioanalysis, click here.
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