Bioanalysis Zone

Robert MacNeill: Various discourses in excellent method performance

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Below you can find links and information on the quarterly column by Robert MacNeill (Envigo), which focuses on quantitative method design.

1. ‘A discourse on sample preparation – Part 1‘ – In this first installment of Robert’s quarterly column, Robert discusses the importance of SPE and details his surprise at the notion held by some that SPE may become obsolete.

2. ‘A discourse on sample preparation – Part 2‘ – In this article Robert discusses the alternatives to SPE for quantitative bioanalysis.

3. ‘A discourse in method robustness – orthogonality of selectivity within a bioanalytical method‘ – In this installment Robert discusses orthogonal selectivity, providing an ironic example of how one assay’s performance was improved by making the selectivity of the SPE closely analogous to the subsequent chromatography.

4. Ruminations on silica-based and organic polymer-based bioanalytical SPE – In this installment of Robert MacNeill’s (Envigo) column, Robert delves into the SPE domain providing an endorsement of silica-based SPE.

5. How analogous can solid-phase extraction and analytical liquid chromatography be? – In this installment of Robert MacNeill’s (Envigo) column, Robert poses the question: How analogous can solid-phase extraction and analytical liquid chromatography be?

6. Solid-phase extraction: a principal option for peptide bioanalytical sample preparation – In this installment of Robert MacNeill’s (Envigo) column, Robert proposes the possibility of using solid-phase extraction for peptide sample preparation accommodating the polar and charged nature associated with peptides.

7. The case for HILIC-SPE – In this installment of Robert MacNeill’s (Envigo) column, Robert makes a case for endeavoring to incorporate orthogonal selectivity in a method even if it means exploring what might be viewed as unorthodox, as long as it’s scientifically sound and ultimately proven with adequate rigor.

8. The specifics of control matrices: How far to go? – In this installment of Robert MacNeill’s (Envigo) column, Robert gives his thoughts about some pertinent aspects of control matrices.

9. Multiple analytes, metabolites and mayhem   – In this installment of Robert MacNeill’s (Envigo) column, Robert discusses the perils and pitfalls of cramming as many analytes as possible, particularly metabolites, into a regulated bioanalytical LC-MS method.

10.Robert MacNeill: Balancing instability precautions against risk of adsorptive losses – In this installment, Robert discusses the instability of drugs within samples and how to balance their instability against absorptive loss.

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